Background: In Ayurveda Classics, Sneha Kalpanas constitutes to be the most widely used and preferred formulations as it incorporates both water and lipid soluble active constituents of the medicine adding to its therapeutic efficacy. Vachalasunadi Taila (VT) is a potent poly herbal formulation explained in the context of Ear Diseases in Sahasrayogam. No research work has been carried out till date and hence an attempt is made to evaluate the pharmaceutico-analytical standardization of VT with special reference to Murchita (MTT) and Amurchitha tila taila (ATT). Methodology: VT was prepared as per the reference of Sharangadhara Samhita. The sample was subjected to different phytochemical parameters like pharmacognostical, physico-chemical and instrumental method of analysis comparing Murchitha (MTT) and Amurchitha (ATT) Tila Taila. Results: Results for parameters includes specific gravity (0.89, 0.91 gm/ml), Refractive Index (1.486, 1.495), acid value (0.44, 0.45), iodine value (111.81, 113.67%), saponification value (187.09, 190.65), ester value (186.65, 190.2). Instrumental analysis HPTLC and GC-MS for MTT and ATT also showed a slight difference. Conclusion: The Pharmaceutico-Analytical standardization of both the samples reveals an improved stability data in all the assessed organoleptic and physico chemical parameters of VTM.