Background: From the time immemorial man is dependent on plant resources to get rid of the ailments. Ishwari (Aristolochia indica Linn.) is a drug having wound healing property used frequently by the folklore practitioners. Non healing ulcer is said to be Dushta vrana routinely encountered in the medical practice were most of the treatment modalities failed to show their extensive efficacy. This is the stage where the practitioners search for the better line of treatment and the efficacious medicines. In folklore practice Ishwarimula kalka is mixed with Nimbu swarasa and applied to the wounds. Hence a clinical research has been taken up to explore the efficacy of root of Ishwari. The aim of the current study is to perform preliminary phytochemical analysis of root of Ishwari (A. indica) and to evaluate its clinical efficacy of in Dushtavrana. Methods: The plant is authenticated referring standard flora; the preliminary phytochemical study was done according to standard protocol. A Single blind comparative randomized clinical trial (RCT) with Pre and Post-test design. 20 patients, diagnosed as Dushta Vrana from the OPD and IPD of SDM. Ayurveda Hospital, Udupi. The Patients were divided into two groups, the Control and the Trial. The Control group treated with H2O2 and Normal saline and bandaged with dry Gauze. In trial group the wound was cleaned with H2O2 and Normal saline and Ishwarimula Kalka prepared by mixing it with Nimbu swarasa was applied. Results: Statistically significant improvement was observed in group treated with Ishwarimula Kalka with Nimbu swarasa. Probably the Tikta, Kashaya Rasa and Teekshna, Ruksha Guna of the drug might have done Samprapti Vighatana of the condition, moreover the test drug was positive for protein, carbohydrate, alkaloid, saponin and other antiseptic content, which has accelerated the process of wound healing. Conclusion: Ishwari Moola Kalka Alepa was observed efficacious in patients of Dushtavrana as an external treatment.